Takže je vymaľované! Súčasný parlament zložený z totálne neschopných politikov skončil. Je úplne namieste povedať, že konečne. Zvádza to konštatovať, že aj fraške je koniec. Lenže… ako možno vidieť z toho, čo sa teraz deje a ako sa títo politici správajú naďalej, vyzerá to skôr na začiatok nového konca. Taký september, aký nás zrejme čaká, nebol ani v Amerike po 11. septembri 2001. Tam spadli len dve budovy, ale u nás hrozí, že padne celý štát… síce len na hubu, ale aj to by malo byť pre obyvateľov Slovenska varovaním.
Stačí sa poobzerať naokolo po predvolebných plagátoch a heslách na nich. Ksichty, čo tu celé roky škodia a neurobili nič z toho, čo nasľubovali predtým, sľubujú ďalej. Jeden hru „mení“, iný ju „nemení“. A „jamkatý“ sľubuje, že všetko bude lacnejšie. Kde bol odteraz? Veď bol aj premiérom! Dokonca vyťahuje kartu o „zodpovednosti politikov“. Doteraz však neprešla a ani nikdy neprejde cez parlament – vlastne cez nich samých. „Hranol“ na „jamkatého“ vyťahuje buzerantstvo a „Balónik“ vyzýva „Hranola“ do súboja, kto má lepších odborníkov. Všimol si azda niekto, aby hocikto iný, mimo SaS, o „Balónikových odborníkoch“ povedal, či napísal, že sú odborníci? Samozrejme, že nie. Toto nehorázne klamstvo šíria o sebe len oni sami . Pripomíname, že ešte nedávno tvrdil, že odbornosť na vrcholných miestach je „na príťaž“, že to všetko musia riadiť „manažéri“. Ale manažér, ktorý o probléme nič nevie, je úplne „nanič“. Viete, ako sa hovorí o odborníkoch a špecialistoch: Špecialista vie všetko o hovne a odborník vie hovno o všetkom. Alebo naopak …? 😊

Ale netýka sa to len „Balónika“, týka sa to všetkých. A netýka sa to len odbornosti, ale najmä toho, kto z nich čo kedy dokázal a najmä ich charakteru. To by mali byť rozhodujúce kritériá na to, aby sme niekomu dovolili nás zastupovať (myslíme tým, v parlamente).
Blíži sa termín ďalších parlamentných volieb a je jedno, či sú predčasné. Znova nás idú tie isté ksichty presviedčať, aby sme volili práve ich. A čo urobí Slovač? Nuž, ako sprosté, tupé stádo pôjde voliť znova tých istých. Veď pamäť nemá žiadnu, sme holubičí národ, ktorý všetko všetkým odpúšťa a tak sme zabudli na výzvu Sama Chalupku ešte z roku 1864 „Mor ho!“. Nemáme už cisára, tak sme si našli na svoje vlastné zotročovanie svojich vlastných ľudí – politikov. Sme ich otrokmi, hračkou v rukách ich moci. Sme presne ako Mimoni zo známeho filmu, ktorí celý čas hľadajú na Zemi toho najhoršieho Zloducha, aby mali koho velebiť a poslúchať. Slovač si tých najhorších zakaždým nájde raz-dva. Nerozumieme, prečo sme my Slováci taký sprostý národ.

Nemá význam rozoberať, koho vlastne voliť. Niet totiž koho. Všetci tu už raz boli a všetci ukázali, že im ide len o ich vlastné blaho. Československo vraj kedysi patrilo medzi najmenej zadlžené štáty. Zvládalo dokonca ekonomicky podporovať Kubu, Vietnam, aj kusisko Afriky. Všetko sme mali zo svojho vlastného vrecka. V pamätnom novembri 1989 sa kričalo, že „Máme holé ruce!“, teraz sa k tomu môžu pridať, že aj „holé rite“. A komu za to vďačíme? Možno by ste povedali, že práve týmto politikom, no nie je to celkom pravda. Vďačíme za to len a len sami sebe, pretože my sme im umožnili robiť si s nami, čo sa im zapáči, či čo sa im v ich chorých hlavách v noci prisnije. Každý robí to, čo sa mu dovolí a my sme im to doteraz dovoľovali. Silne pochybujeme, že by sa to po nadchádzajúcich voľbách zmenilo.

Totiž, každý národ má takú vládu, akú si zaslúži… alebo horšiu.

Tento portál mal slúžiť na to, aby si náš národ uvedomil, kde je skutočná podstata jeho vlastnej biedy, kde sú korene toho zla a aké sú možné cesty z tohto marazmu von. Nezdá sa nám, že by sme boli dosiahli, čo sme zamýšľali. Podľa všetkého sa tu nič nezmení, lebo ľuďom je už všetko jedno.

Ale pamätajte si, nikto nám neurobí tak zle, ako my sami sebe. Na to pamätajte, keď pôjdete v septembri k volebným urnám.

moderátori portálu

38 011 Replies to “Parlament … a fajka zhasla“”

  1. In the context of Fmoc SPPS, service and solid‐phase methods to farnesylation are nicely exhibited by the adhering to reported syntheses of yeast mating pheromone a‐factor. The synthesis of this peptide is made complex by the fact that it has not just a farnesyl group however likewise a C‐terminal cysteine methyl ester. One restriction of the benzyl‐type defense is the development of long‐lived benzyl cations throughout deprotection that can respond with the peptide, a specific concern with tryptophan. Additionally, on some long peptides and near‐basic deposits, long term acidolysis is needed for elimination.

    From 2016 to 2022, the FDA accepted 26 peptides as medicines, resulting in over 315 new peptide medications authorized in the exact same timeframe. Peptides are currently used to deal with a number of pathologies, from microbial infections to obesity to cancer cells. GLP-1 agonists are likewise a course of peptide-based medicines.

    On the other hand, electrophoresis is extensively made use of by biochemists and can supply a basic picture of both the dimension of your target healthy protein whether various other protein-based impurities present. Peptide purity is routinely identified by HPLC (high efficiency fluid chromatography ). This approach makes it possible for separation and loved one metrology of each peptide synthesized. If paired to a mass spectrometer(MS), this provides molecular mass/ identity information of the target peptide and going along with contaminations. associated impurity, the degree for the recommended common synthetic peptide is no more than 0.5 percent of the medicine material.

    Lyophilized peptides consist of pollutants in the type of counterions and residual water. Internet peptide web content, measured by amino acid evaluation, is the percentage of all peptides relative to these non-peptide impurities. It varies according to the filtration and lyophilization treatments, and is impacted by the amino acid make-up, particularly the visibility of hydrophilic amino acids in the sequence. After all, there are 2 different, focus dependent, bimolecular responses that need to be finished in order to add a solitary amino acid to a growing peptide chain.
    3 Peptide Synthesis By Piece Ligation
    Although we introduced a treatment for cleaving 24 peptides in parallel, further optimization is required to apply this procedure on a larger range. A second challenge is restricted peptide solubility, which usually delayed purification. A third difficulty is peptide filtration by preparative RP-HPLC, which in some cases needs numerous rounds to accomplish high pureness (≥ 95% pureness). Although these difficulties are a standard component of peptide manufacturing, they can postpone the manufacturing of neoantigen vaccinations and eventually hold off person therapy. Creative remedies to these obstacles are quickly needed to completely resolve the peptide manufacturing and scalability issues for personalized neoantigen vaccinations.
    Table 1
    With respect to hydrogels, batch-to-batch uniformity can be attained utilizing peptides of high pureness. In this chapter, solid-phase peptide synthesis is discussed in the context of the β-hairpin peptide hydrogels examined in our lab (25,38– 42). Guidelines for the filtration and key characterization of the peptides are offered together with a short conversation of select strategies utilized in the biophysical, architectural, and mechanical characterization of the hydrogels. An example of the implications of purity on the material residential properties is likewise provided.
    Nonetheless, when evaluating through the literary works, regrettably no special method is available for the synthesis of extremely hydrophobic „challenging“ peptides and proteins. Nowadays, for each brand-new designated synthesis, special optimization of SPPS, NCL and follow-up procedures are still called for. The „hard sequence“ concept has been introduced in the 80’s and was offered distinction by Kent and colleagues for peptides that form strong inter- or intra molecular, non-covalent communications which develop insoluble peptide aggregates. „Challenging series“ are peptide series which contain high variety of amino acids having hydrophobic side chains, so-called β-branched amino acids, consisting of leucine, valine, phenylalanine or isoleucine. In addition, glycine is known to generate β-sheet packaging in combination with afore mentioned amino acids (Paradis-Bas et al., 2016).

    Extra Information
    Just like other DNA synthesis techniques, the prolongation cycle performances are the restricting element. In each response site, a percent of insufficient thermolysis of PGs is expected. With every elongation cycle, removal sequences would accumulate developing impurities similar to the wanted item. Evonetix resolved this concern by tethering each paralyzed oligonucleotide to the chip by means of a linker that is labile to thermally assisted chemical cleavage. Once the preferred strands 53 are constructed, the website on the chip to which they are incapacitated is warmed causing the cleavage of the linker and the liberation of these hairs right into service. These liberated hairs can be made corresponding to oligonucleotides 52, which stay paralyzed on the chip and can be ultimately annealed with each other to yield double-stranded DNA particles 54 (ref. 150).

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